THE INFLUENCE OF SELF-ORGANIC COMPOUNDS ON CLINICAL AND METABOLIC MANIFESTATIONS 
IN MALE BALb/c LINES AT COBALT COVERAGE

Lovtsova Larisa Gennadievna, Candidate of engineering sciences, associate professor, sub-department of microbiology, biotechnology and chemistry, Saratov State Vavilov Agrarian University (1 Teatralnaya square, Saratov, Russia), Larisalovtsova2009@rambl
Guliy Ol'ga Ivanovna, Doctor of biological sciences, associateprofessor, professor at the sub-department of microbiology, biotechnology and chemistry, Saratov State Vavilov Agrarian University (1 Teatralnaya square, Saratov, Russia),  guliy_olga@mail.ru
Drevko Yaroslav Borisovich, Candidate of chemical science, associate professor, sub-department of microbiology, biotechnology and chemistry, Saratov State Vavilov Agrarian University (1 Teatralnaya square, Saratov, Russia), drevko@list.ru
Kozlov Sergey Vasil'evich, Candidate of veterinary sciences, associateprofessor, sub-department of animaldiseases and veterinary-sanitary expertise,Saratov State Vavilov Agrarian University (1 Teatralnaya square, Saratov, Russia), kozlovsv12@yandex.ru
Smutnev Petr Vladimirovich, Candidate of veterinary sciences, associate professor, sub-department of microbiology, biotechnology and chemistry, Saratov State Vavilov Agrarian University (1 Teatralnaya square, Saratov, Russia), smutnev-asd@yandex.ru

57.017

DOI

10.21685/2307-9150-2018-1-8

Background. A significant impact on living organisms and their communities is provided by man-made inputs of various heavy metals including cobalt. The aim of our work was to study the prophylactic efficacy of organoselenium compounds (2,4-diphenyl-tetrahydro-selenochromen and 2-phenyl-4-(p-bromo-phenyl)-tetrahydroselenochromen) on the model of acute poisoning with cobalt chloride in BALb/c mice.
Materials and methods. Biochemical studies, when considering the effect of two organoselenium compounds on the metabolic parameters of blood in mice, were performed on a biochemical analyzer “StatFax 3300” using the diagnostic systems of
the firm “Olvex Diagnosticum” and “Diakon DS”. To check the correctness and accuracy of the determination of biochemical parameters in serum, a control serum for biochemical studies was used in accordance with TU 9398-022-09807247-2009,
LLC “HOSPITEX DIAGNOSTICS”.
Results. As a result of the conducted studies, it has been established that the introduction of organoselenium compounds (2,4-diphenyl-tetrahydro-selenochromene and 2-phenyl-4-(p-bromo-phenyl)-tetrahydroselenochrome) inhibits the development
of the pathological process in BALb/c mice exposure to inflated doses of xenobiotic, cobalt chloride.
Conclusions. It was found that the best indices for decreasing intoxication of the salt of this metal shows 2-phenyl-4-(p-bromo-phenyl)-tetrahydroselenochrome. Presumably because of the structure of the molecule with the introduction of Br into
the paraposition, at which the electron density is mixed with Br and, as a consequence, Se becomes more reactive. Along with this, it was reliably established that the oral administration of organoselenium compounds (2,4-diphenyl-tetrahydroselenochrome
and 2-phenyl-4-(p-bromo-phenyl)-tetrahydroselenochromes) at doses of 3,8 and 3,2 mg/kg, a clinically healthy animal does not have a negative effect on the vital activity of the mice.

Key words

cobalt, detoxification, metabolism, selenium compounds, biochemical blood test, xenobiotic, heavy metal poisoning

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